0.70处为cha斑点（蓝色荧光），随着醇沉浓度的增加，杂质含量逐渐减少，当醇沉浓度达90%时，几乎
0.70 chu wei cha ban dian lan se ying guang sui zhe chun chen nong du de zeng jia za zhi han liang zhu jian jian shao dang chun chen nong du da 90% shi ji hu
的乙醇103ml，216ml，760ml，cha损失率仅为11.7%.由纸层析图谱可知，紫外灯下rf
de yi chun 103ml 216ml 760ml cha sun shi lv jin wei 11.7%. you zhi ceng xi tu pu ke zhi zi wai deng xia rf
至90%时，cha损失率为16.4%.若采用梯度递增方式醇沉即60%～80%～90%，依次加入95%
zhi 90% shi cha sun shi lv wei 16.4%. ruo cai yong ti du di zeng fang shi chun chen ji 60% 80% 90% yi ci jia ru 95%
损失率为6.5%;若用240ml无水乙醇醇沉至80%时，cha损失率9.8%;540ml无水乙醇醇沉
sun shi lv wei 6.5%; ruo yong 240ml wu shui yi chun chun chen zhi 80% shi cha sun shi lv 9.8%;540ml wu shui yi chun chun chen
min，浸提3次，平均浸出率93.82%，加90ml无水乙醇醇沉至60%（溶液中乙醇浓度）时，cha
min jin ti 3 ci ping jun jin chu lv 93.82% jia 90ml wu shui yi chun chun chen zhi 60% rong ye zhong yi chun nong du shi cha
h值，减压干燥得粗品氯原酸，纸层析法分析其纯度. 2 结果 用6倍于杜仲叶重量的水，50℃下40
h zhi jian ya gan zao de cu pin lv yuan suan zhi ceng xi fa fen xi qi chun du . 2 jie guo yong 6 bei yu du zhong ye zhong liang de shui 50 xia 40
定含量.滤液加饱和醋酸铅至不再生成沉淀为止，滤取沉淀，水洗，10%h2so4脱铅，活性炭脱色，调节p
ding han liang . lv ye jia bao he cu suan qian zhi bu zai sheng cheng chen dian wei zhi lv qu chen dian shui xi 10%h2so4 tuo qian huo xing tan tuo se diao jie p
缩至6xiao77论坛唯美清纯0ml，紫外分光光度法测定含量［1］.滤液分别进行一次醇沉和分次醇沉，除去蛋白质多糖等杂质，测
suo zhi 6xiao77 lun tan wei mei qing chun 0ml zi wai fen guang guang du fa ce ding han liang 1 . lv ye fen bie jin hang yi ci chun chen he fen ci chun chen chu qu dan bai zhi duo tang deng za zhi ce
趁热过滤，滤渣加水600ml，重复作，第三次加水400ml，50℃下浸提30min，合并滤液，减压浓
chen re guo lv lv zha jia shui 600ml zhong fu zuo di san ci jia shui 400ml 50 xia jin ti 30min he bing lv ye jian ya nong
燥后搓成粗粉，过20目筛，称取杜仲叶粗粉4份各100g，分别加xiao77论坛唯美清纯水600ml，50℃下浸提40min，
zao hou cuo cheng cu fen guo 20 mu shai cheng qu du zhong ye cu fen 4 fen ge 100g fen bie jia xiao77 lun tan wei mei qing chun shui 600ml 50 xia jin ti 40min
醇-乙酸-水（4∶1∶5）上清液.新华1号层析纸.所用试剂均为分析纯. 1.2 方法杜仲叶60℃干
chun - yi suan - shui 4 1 5 shang qing ye . xin hua 1 hao ceng xi zhi . suo yong shi ji jun wei fen xi chun . 1.2 fang fa du zhong ye 60 gan
.氯原酸标准品由美国进口.日本岛津uv-265自动记录分光光度计.zf-2型三用紫外仪.展开剂:正丁
. lv yuan suan biao zhun pin you mei guo jin kou . ri ben dao jin uv-265 zi dong ji lu fen guang guang du ji .zf-2 xing san yong zi wai yi . zhan kai ji : zheng ding
的影响，为后续cha分离纯化打xiao77论坛唯美清纯下基础. 1 材料和方法 1.1 材料杜仲叶由陕西省城固县科委提供
de ying xiang wei hou xu cha fen li chun hua da xiao77 lun tan wei mei qing chun xia ji chu . 1 cai liao he fang fa 1.1 cai liao du zhong ye you shan xi sheng cheng gu xian ke wei ti gong
.因此我们尝试了水提醇沉工艺，着重研究了一次醇沉及以梯度递增方式醇沉（分次醇沉）对水提液中cha含量
. yin ci wo men chang shi le shui ti chun chen gong yi zhe zhong yan jiu le yi ci chun chen ji yi ti du di zeng fang shi chun chen fen ci chun chen dui shui ti ye zhong cha han liang
、沉淀萃取xiao77论坛唯美清纯及hplc分离纯化，这些方法不但成本高而且工序复杂.cha分子中有五个羟基，有一定的水溶性
chen dian cui qu xiao77 lun tan wei mei qing chun ji hplc fen li chun hua zhe xie fang fa bu dan cheng ben gao er qie gong xu fu za .cha fen zi zhong you wu ge qiang ji you yi ding de shui rong xing
一个新途径.目前国内外对氯原酸的提取分离多以咖啡豆及金银花为原料，甲醇、乙醇等有机溶剂提取，用柱层析
yi ge xin tu jing . mu qian guo nei wai dui lv yuan suan de ti qu fen li duo yi ka fei dou ji jin yin hua wei yuan liao jia chun yi chun deng you ji rong ji ti qu yong zhu ceng xi
同，且叶中cha含量明显高于皮.若以叶为原料xiao77论坛唯美清纯提取cha，不但可变废为宝，而且为杜仲叶的综合利用开辟了
tong qie ye zhong cha han liang ming xian gao yu pi . ruo yi ye wei yuan liao xiao77 lun tan wei mei qing chun ti qu cha bu dan ke bian fei wei bao er qie wei du zhong ye de zong he li yong kai pi le
毒、补中益气、镇痛强志、消炎利胆的主要有效成份.杜仲传统仅以皮入药，近来研究证实杜仲叶和皮成分基本相
du bu zhong yi qi zhen tong qiang zhi xiao yan li dan de zhu yao you xiao cheng fen . du zhong chuan tong jin yi pi ru yao jin lai yan jiu zheng shi du zhong ye he pi cheng fen ji ben xiang
贵补益中药杜仲的主要活性成分之一xiao77论坛唯美清纯，对多种致病菌和病毒有较强的抑制和杀灭作用，是众多药材和中成药抗菌解
gui bu yi zhong yao du zhong de zhu yao huo xing cheng fen zhi yi xiao77 lun tan wei mei qing chun dui duo zhong zhi bing jun he bing du you jiao qiang de yi zhi he sha mie zuo yong shi zhong duo yao cai he zhong cheng yao kang jun jie
关键词:杜仲叶;氯原酸;提取;醇沉 0引言 氯原酸（chlorogenicacid，cha）是名
guan jian ci : du zhong ye ; lv yuan suan ; ti qu ; chun chen 0 yin yan lv yuan suan chlorogenicacid cha shi ming